Background There has been no systematic study assessing nickel release from surgical instruments and equipment used within the operating suite. This equipment represents important potential sources of exposure for nickel-sensitive patients and hospital staff. OBJECTIVE: To investigate nickel release from commonly used surgical instruments and operating room equipment. METHODS AND MATERIALS: Using the dimethylglyoxime nickel spot test, a variety of surgical instruments and operating room equipment were tested for nickel release at our institution. RESULTS: Of the 128 surgical instruments tested, only 1 was positive for nickel release. Of the 43 operating room items tested, 19 were positive for nickel release, 7 of which have the potential for direct contact with patients and/or hospital staff. CONCLUSION: Hospital systems should be aware of surgical instruments and operating room equipment as potential sources of nickel exposure.